When a web application is secured by the DualShield IIS Agent with MFA, the agent adds an extra layer of authentication over the web application's form-based authentication. Without Single-Sign-On enabled, users will be first authenticated by the DualShield SSO, then by the web application's original login process that's usually the user's AD credential verification.
You have 2 options:
From the security point of view, both options have no difference. However, from the user experience point of view, option 2 will deliver a more coherent user experience.
Furthermore, DualShield IIS Agent provides 2 options for implementing Single Sign-On
Between Auto-Filling and Auto-Post options, Auto-Post is preferred as it is easier to set up and quicker in performance. However, some IIS web servers have such restrictions that it is not possible to enable Single Sign-On by Auto-Post